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Module TSS3: CAGE, RAMPAGE, and RNA Pol II X-ChIP-Seq

August 15, 2020January 3, 2025

This module uses the placement of the Initiator (Inr) motif, TSS annotations produced by the Celniker group at modENCODE, and experimental data such as CAGE, RAMPAGE, and RNA Polymerase II X-ChIP-Seq data to characterize the promoter for the Antp gene in D. melanogaster.

Module TSS2: Using Sequence Alignment to Identify a TSS

August 15, 2020January 3, 2025

This module illustrates how pairwise (blastn) and multiple sequence alignments can be used in conjunction with RNA-Seq data and the Short Match functionality of the UCSC Genome Browser to facilitate the TSS annotation of the Antp gene in D. eugracilis.

Module TSS1: Transcription Start Sites Introduction

August 15, 2020January 3, 2025

This module describes how Celniker TSS annotations, DNase I hypersensitive sites, and the 9-state chromatin models can be used in the classification of promoter shape for the Antp gene.

Module 6. Alternative splicing

August 15, 2020January 3, 2025

This module explores how multiple different mRNAs and polypeptides can be encoded by the same gene. After completing this module students will be able to explain how alternative splicing of a gene can lead to different mRNAs and illustrate how alternative splicing can lead to the production of different polypeptides and result in drastic changes in phenotype.

Module 5. Translation: The need for an Open Reading Frame

August 15, 2020January 3, 2025

In this module students will learn how mRNA is translated into a string of amino acids. After completing this module students will be able to determine the codons for specific amino acids as well as start and stop codons. They will be able to identify open reading frames for a given gene, define the phases of splice donor and acceptor sites and describe how they impact the maintenance of the open reading frame.

Module 4. Removal of introns from pre-mRNA by splicing

August 15, 2020January 3, 2025

This module uses mRNA data to identify splice sites. After completing this module students will be able to identify intron-exon boundaries using canonical splice donor and acceptor sequences and determine which are best supported by RNA-Seq and TopHat splice junction predictions.

Module 3. Transcription, Part II: What happens to the initial transcript made by RNA pol II?

August 15, 2020January 3, 2025

This module demonstrates how the transcript generated by RNA polymerase II (the pre-mRNA) is processed to become mature mRNA using the sequence signals identified in Module 2. After completing this module students will be able to use the genome browser to explain the relationships among pre-mRNA, 5′ capping, 3′ polyadenylation, splicing, and mRNA.

Module 2. Transcription, Part I: From DNA sequence to transcription unit

August 15, 2020January 3, 2025

This module illustrates how a primary transcript (pre-mRNA) is synthesized using a DNA molecule as the template. After completing this module students will be able to explain the importance of the 5′ and 3′ regions of the gene for initiation and termination of transcription by RNA polymerase II, and identify the beginning and end of a transcript using the capabilities of the genome browser (RNA-Seq, Short Match).

Module 1. Introduction to the Genome Browser: What is a gene?

August 15, 2020January 3, 2025

This module introduces students to the UCSC Genome Browser. After completing this module students will be able to navigate to a genomic region and to control the display setting for different evidence tracks. Additionally, students will be able to explain the relationships among DNA, pre-mRNA, and protein.

Gene Finding in Chimpanzee

August 15, 2020September 2, 2022

A step-by-step tutorial that takes the student through a gene identification problem in a chimpanzee BAC sequence using predictions from a gene finder (Genscan), the UCSC Browser and BLAST.

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