An introductory exercise using BLAST to annotate a region in the Drosophila melanogaster genome. Students can use this exercise to gain familiarity with performing BLAST searches and interpreting BLAST output. An answer key is provided for instructors.
This lab is an updated, stand-alone version of the bioinformatics exercise originally developed for Bio 3055 at Washington University. This exercise introduces students to the genetic basis of disease using the KRas protein.
Students use grasshoppers collected from glades in the Missouri Ozarks to generate and analyze sequence data to determine if the grasshopper populations are becoming fragmented due to the suppression of forest fires. Sequence data are provided here so students who do not have access to grasshoppers can still perform the sequence analysis portion of the lab.
Students use two different protocols, one based on sequence analysis and one based on biochemistry, to determine the identity of an unknown bacterial strain.
This lab provides an opportunity for students to randomly clone a fragment of the yeast genome into E. coli and then investigate what part of the genome they have cloned through sequence analysis.