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Drosophila

Module 2. Transcription, Part I: From DNA sequence to transcription unit

This module illustrates how a primary transcript (pre-mRNA) is synthesized using a DNA molecule as the template. After completing this module students will be able to explain the importance of the 5′ and 3′ regions of the gene for initiation and termination of transcription by RNA polymerase II, and identify the beginning and end of a transcript using the capabilities of the genome browser (RNA-Seq, Short Match).
Exercise
Answer Sheet
Answer Key
Exercise
Answer Sheet
Answer Key

Using FlyBase RNA-Seq Tools to Investigate Gene Expression Profiles

This walkthrough illustrates how GBrowse and the RNA-Seq tools at FlyBase can be used to identify genes with similar expression patterns. This walkthrough provides an introduction to the TopoView RNA-Seq track in FlyBase GBrowse, the FlyBase Expression Profile Search tool, and the Expression Similarity Search tool. It also illustrates how the Gene2Function web site can be used to infer the function of a D. melanogaster gene based on the functions of its orthologs in other model organisms.

Web Databases for Drosophila

This PowerPoint presentation introduces the species-specific databases available on the web, including FlyBase and the Ensembl Drosophila melanogaster database.

Quick Check of Student Annotations

This document describes a protocol that GEP faculty can use to quickly identify potential errors in student gene annotations.

D. mojavensis: Issues of Polymorphisms

This PowerPoint presentation discusses the potential sequence improvement issues caused by single nucleotide and insert polymorphisms in the D. mojavensis and D. grimshawi projects.