This document contains advice on the factors that students should consider when designing PCR primers to resolve gaps and low quality regions. The document also includes the specific search parameters students should use to minimize the chance of off-target priming when designing PCR primers.
This document describes a protocol that GEP faculty can use to quickly identify potential errors in student gene annotations.
Three sample chimp chunk projects with student reports.
Following the step-by-step tutorial “Chimp BAC Analysis: Genes and Pseudogene”, students should be ready to annotate a chunk of chimpanzee DNA themselves, presenting evidence to support their claim of a gene or pseudogene. An example student report from the Bio 4342 course at WU is available here.
Brief guidelines with advice on annotating chimpanzee DNA, including what to put in the final report.
