This lab is an updated, stand-alone version of the bioinformatics exercise originally developed for Bio 3055 at Washington University. This exercise introduces students to the genetic basis of disease using the KRas protein.
Students use two different protocols, one based on sequence analysis and one based on biochemistry, to determine the identity of an unknown bacterial strain.
This lab provides an opportunity for students to randomly clone a fragment of the yeast genome into E. coli and then investigate what part of the genome they have cloned through sequence analysis.
Students use the HIV Problem Space on the BioQuest BEDROCK Website to investigate whether a specific HIV mutation can be correlated with a decline in immune system function. In order to perform this analysis, students must generate and analyze multiple sequence alignments of HIV sequences generated from the ALIVE study.