This lecture on Long-Read Sequencing Technology was created by Katie Sandlin for the 2024 National GEP Faculty Workshop. The lecture covers four major topics: 1) Review of RNA-Seq Short-read Sequencing, 2) Overview and benefits of Long-read Sequencing, 3) Oxford Nanopore Sequencing, and 4) Pacific Biosciences Sequencing. Each slide is scripted in the Notes section for reference.