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Bob Fulton

Dye Chemistry Characteristics

This document illustrates some of the advantages and potential problems with the BigDye and dGTP chemistries.

Fulton Lecture Notes

The lecture notes from a presentation by Bob Fulton point out some of the more advanced features of Consed, the base caller phred, and the assembler phrap. This document should be read after students have some experience using Consed but before they begin working on their fosmids.

Restriction Digests: A Tool to Check Your Findings

One of the main tools used to verify the correctness of a genome assembly is comparison of an in-silico restriction digest to the real restriction digest data generated by the WU Genome Center. This document also covers how students can use the restriction digest data early in the assembly process to determine the number of copies of repeats in a given cluster or to estimate gap sizes.