Pathways Project: Annotation Videos
This series of videos is intended to help GEP students annotate a Pathways Project gene from start to finish.
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Pathways Project: Annotation Form D. pseudoobscura Key
Students can apply what they learned in the Pathways Project: Annotation Walkthrough to construct a gene model for Rheb in D. pseudoobscura by completing the Pathways Project: Annotation Form. An answer key (generic username and password required) is provided to assist instructors in checking the accuracy of the annotation and includes potential areas of confusion throughout.
Pathways Project: Annotation Form Exemplar
The Annotation Form Exemplar is provided as an example of a completed Annotation Form ready for submission to the GEP’s Pathways Project. The optional questions were omitted from the exemplar.
Pathways Project: Annotation Form
This “Annotation Form” merged the “Annotation Report” and “Annotation Notebook” into a single document and the latter two items were archived.
The “Annotation Form” kept many of the Checks for Understanding type questions that were previously in the “Annotation Notebook.” However, given that not all faculty want their students to answer those questions, we marked any question that is NOT required for submission to the GEP as “OPTIONAL.” Currently the optional questions are organized as letters rather than numbers, which we hope will make it easy to quickly select and delete the OPTIONAL questions that faculty don’t wish to include. Again, all numbered questions are required for submission to the GEP for reconciliation.
Items that were previously asked for in the “Project Details” table of the “Annotation Report” are now available on the Genome Browser Gateway page for each species. Therefore, the “Project Details Table” instructions document is no longer needed.
4. Characteristics of the F Element
This lecture is Part 4 of a 4-part series—F Element Project: Annotated Lecture Slides.
This lecture uses the themes from Slide Sets 1-3, of the “F Element Project: Annotated Lecture Slides” sequence, in describing what we have learned about the F element—combining wet-bench work in the Elgin lab, results of chromatin mapping by the modENCODE consortium, and the bioinformatics efforts of the faculty and students of the GEP. This includes the following:
- characterization of the F element as a heterochromatic domain, high in repeated DNA but nonetheless having genes expressed at normal levels (7 slides);
- mapping the chromatin state in relationship to the genes and to reporter transgenes inserted into the F (9 slides);
- exploring the TSS and searching for unique factors or motifs associated with the TSSs of F element genes (6 slides);
- introducing the “expanded F” project, describing some of the finds made looking at the F of D. ananassae, and summarizing the ongoing challenges and questions (9 slides)
There is very significant overlap between this lecture and another.
One or the other can be used—either the all-in-one lecture, “Why study the F Element? or the series of four lectures titled F Element Project: Annotated Lecture Slides sequence —but not both.
One or the other can be used—either the all-in-one lecture, “Why study the F Element? or the series of four lectures titled F Element Project: Annotated Lecture Slides sequence —but not both.
3. The Dilemma of Transposable Elements: Can’t Live with Them, Can’t Evolve without Them!
This lecture is Part 3 of a 4-part series—F Element Project: Annotated Lecture Slides.
This lecture introduces students to the analysis of repetitious elements in the genome. It can be used as a stand-alone lecture, or since the content is also important to our thinking about the relationship of transposable elements to eukaryotic genomes which is a key issue in our study of the expanded F element, it can be included in the “F Element Project: Annotated Lecture Slides” sequence.
The slides provide each of the following:
- an introduction to transposable elements (TEs), particularly in the human genome (5 slides),
- examples showing how transposition, resulting in new insertion sites or new rearrangements, creates harmful mutations and/or stimulates inflammation (7 slides);
- how mechanisms for silencing generate more options for gene regulation (4 slides);
- how transposable elements have rewired the genome, contributed to novel regulatory proteins, helped build centromeres and telomeres, marked sex chromosomes, and might drive evolution during times of stress (7 slides)
Without our TEs and histones, we would be bacteria!
2. Heterochromatin Formation — It’s all about silencing!
This lecture is Part 2 of a 4-part series—F Element Project: Annotated Lecture Slides.
This lecture develops the relationship between chromatin packaging and control of gene expression, a significant epigenetic system that allows the genome to respond to changes in environment, both the external environment and physiological cues (e.g., hormone responses). There are 7 slides developing the importance of epigenetic regulation, particularly the silencing of repeats by heterochromatin packaging; 6 slides on histone post-translational modification; 9 slides on the discovery of Heterochromatin Protein 1a and its validation using Position Effect Variegation (in Drosophila), including the model for spreading of heterochromatin; 8 slides on HOW heterochromatin packaging can lead to silencing; 3 slides on the inheritance and manipulation of the heterochromatic state; and 4 slides on mapping chromatin states across the fly genome.
1. Eukaryotic Genomes and Chromatin Structure
This lecture is Part 1 of a 4-part series—F Element Project: Annotated Lecture Slides.
This lecture introduces the following topics:
Development of the nucleosome model represents a paradigm shift in our thinking about chromosomes, and slides are included pointing out how this model was initially rejected, but subsequently achieved widespread support in the scientific community.
- C-value paradox (2 slides)
- explains how we first recognized that eukaryotic genomes are full of repetitious sequences by using Cot curves (11 slides; allows you to remind students about second order rate equations they learned in freshman chemistry!);
- repeat characteristics of eukaryotic genomes (5 slides);
- the need to package all that DNA to get it into a nucleus (3 slides);
- the development of the nucleosome model (11 slides);
- the relationship between nucleosome arrays and gene expression (4 slides).
Development of the nucleosome model represents a paradigm shift in our thinking about chromosomes, and slides are included pointing out how this model was initially rejected, but subsequently achieved widespread support in the scientific community.
Why Study the F Element?
This video provides a 50-minute talk on our motivation and progress for the F Element “expansion” Project. The talk briefly introduces the
- C-value paradox (2 slides);
- the need to silence repeats (3 slides);
- basic chromatin structure (3 slides);
- heterochromatin and the F Element (6 slides);
- mapping the F Element in D. melanogaster for repeats and heterochromatin structure (10 slides);
- examining the Transcription Start Site, looking for regulatory motifs (6 slides);
- describing the “F Element expansion” project and our initial findings (8 slides).
The slide set used in the video is provided as a PowerPoint (automatic download) and a PDF Handout using the buttons below.
Parasitoid Wasps Project: Annotation Workbook
For the Parasitoid Wasps Project gene annotation, project submission will consist of a completed Annotation Workbook, a screenshot of the user track on the Genome Browser, and sequence files for the entire transcript and encoded protein. This Annotation Workbook is an Excel file with space to fill out all the necessary information for the gene annotation.
If it is done correctly, filling out the workbook will also auto-populate a GFF file that can be used for testing the accuracy of gene models (described in the “Checking Accuracy of the Gene Model (Alternative to Gene Model Checker)” section of the “Annotation Workbook Directions“). Please note that everything should be filled out exactly as described: the vocabulary and formatting need to be very precise to make a functional GFF file.
The “Example” sheet has been completed for you to reference while completing the “Transcript” sheet.
