This lecture introduces the C-value paradox and explains how we first recognized that eukaryotic genomes are full of repetitious sequences by using Cot curves; followed by repeat characteristics of eukaryotic genomes; the need to package all that DNA to get it into a nucleus; the development of the nucleosome model; and the relationship between nucleosome arrays and gene expression.
Annotated Lecture Slides
F Element Project
Each PowerPoint contains extensive notes, bringing out points instructors might want to make in presenting the material, adding details for the experiment being shown, clarifying nomenclature, etc. In many cases the DOI or a link to the paper being cited is included in the notes. If you would like to access a specific paper, and cannot do so from your library, contact Dr. Sarah C.R. Elgin (Washington University in St. Louis).
These lectures assume that the students have a good grasp of the material in the “Understanding Eukaryotic Genes” modules.
Single use of a copyright figure from the literature for teaching is permitted if the citation is included.
This video provides a 50-minute talk on our motivation and progress for the F Element Project. The talk briefly introduces the C-value paradox (2 slides); the need to silence repeats (3 slides); basic chromatin structure (3 slides); heterochromatin and the F Element (6 slides); mapping the F Element in D. melanogaster for repeats and heterochromatin structure (10 slides); examining the Transcription Start Site, looking for regulatory motifs (6 slides); describing the “F Element expansion” project and our initial findings (8 slides).
To develop these concepts in more depth, the following four slide decks are available. Each is intended for a one-hour lecture, although longer will be needed if the concepts are entirely new to the students.
This lecture develops the relationship between chromatin packaging and control of gene expression, a significant epigenetic system that allows the genome to respond to changes in environment, both the external environment and physiological cues (e.g., hormone responses).
This lecture introduces students to the analysis of repetitious elements in the genome. It can be used as a stand-alone lecture, or included in the “F Element Project: Annotated Lecture Slides” sequence of lectures.
This lecture combines wet-bench work in the Elgin lab, results of chromatin mapping by the modENCODE consortium, and the bioinformatics efforts of GEP faculty and students to describe what we have learned about the F element.