This module illustrates how pairwise (blastn) and multiple sequence alignments can be used in conjunction with RNA-Seq data and the Short Match functionality of the UCSC Genome Browser to facilitate the TSS annotation of the Antp gene in D. eugracilis.
Module TSS2: Using Sequence Alignment to Identify a TSS
- Last Reviewed: August 19, 2024
- Last Updated: August 19, 2024
- Meg Laakso
- Created: February 17, 2018
- Curriculum Section: Gene Annotation, TSS Annotation
- Curriculum Type: Lesson with Exercises
- Target Audience: Beginning Student
- NIBLSE Competencies: C5
- Topic Tags: Multiple Sequence Alignment, RNA-Seq, Similarity Search, TSS Annotation
- Tool Tags: BLAST, Short Match, UCSC Genome Browser