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Sequence Homology

Browser-Based Annotation and RNA-Seq Data

  • August 15, 2020January 2, 2022

This exercise continues your introduction to practical issues in comparative annotation. You will be annotating genomic sequence from the dot chromosome of Drosophila mojavensis using your knowledge of BLAST and some improved visualization tools. You will also consider how best to integrate information from high-throughput sequencing of expressed RNA.

Using mRNA and EST Evidence in Annotation

  • August 15, 2020January 2, 2022

This exercise allows students to learn how to use mRNA and Expressed Sequence Tags (ESTs) evidence to assist their annotation efforts. An answer key is available for instructors.

Detecting and Interpreting Genetic Homology

  • August 15, 2020January 2, 2022

An introductory exercise using BLAST to annotate a region in the Drosophila melanogaster genome. Students can use this exercise to gain familiarity with performing BLAST searches and interpreting BLAST output. An answer key is provided for instructors.

Using BLAST for Genomic Sequence Annotation

  • August 15, 2020January 2, 2022

Similar to the Lecture Notes on Alignment, this is a PowerPoint presentation given by Dr. Jeremy Buhler for the GEP faculty and TA workshops. This presentation covers the basics of alignment, essential for students to correctly interpret BLAST results.